Comparison of histological subtypes and Human Papillomavirus (HPV) genotypes in penile intraepithelial neoplasia (PeIN) with and without invasive squamous cell carcinoma: a study of 76 lesions in 43 patients using laser capture microdissection and pcr
DOI:
https://doi.org/10.32480/rscp.2026.e3118Keywords:
Penile neoplasia, penile squamous cell carcinoma, penile intraepithelial neoplasia, human papillomavirus, laser microdissectionAbstract
Penile intraepithelial neoplasia (PeIN) and invasive penile carcinoma present a bimodal profile: HPV-independent and HPV-associated. PeIN may occur as a solitary lesion or in association with invasive carcinoma. This study compares histological subtypes and HPV genotypes across both groups. We evaluated PeIN without invasion (40 lesions from 17 patients) and PeIN associated with invasive carcinoma (36 lesions from 26 patients). Lesions were classified as HPV-independent (differentiated), HPV-associated (basaloid, warty-basaloid, and warty), or mixed. Each lesion was microdissected using the LCM and HPV genotyping was performed with the SPF-10/DEIA/LiPA25 system. Differentiated PeIN was significantly more frequent in PeIN with invasive SCC. Conversely, HPV-associated PeIN was more common in solitary PeIN. A greater diversity of HPV genotypes was detected in PeIN alone (15 vs 6 genotypes). Genotypes exclusive to solitary PeIN included HPV 30, 33, 35, 39, 44, 58, 66, 73, 84, 87, 51, and 59. Genotypes exclusive to PeIN with carcinoma were HPV 11, 18, and 74. HPV 16, 52, and 56 were detected in both groups. The lack of correspondence between histological subtypes and HPV genotypes detected in PeIN alone and PeIN with invasive carcinoma suggests that a number of solitary PeIN lesions do not progress to invasive carcinoma.
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