Analysis of the activity of oncocalyxone A (Auxemma oncocalyx) and doxorubicin on the in vitro development of porcine oocytes

Authors

  • Johanna Leiva Revilla Universidade Estadual do Ceará, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza-CE, Brazil
  • Carolina Maside Universidade Estadual do Ceará, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza-CE, Brazil
  • Luis Vieira Universidade Estadual do Ceará, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza-CE, Brazil
  • Jesús Cadenas Universidade Estadual do Ceará, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza-CE, Brazil
  • Ana Clara Ferreira Acioly Universidade Estadual do Ceará, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza-CE, Brazil
  • Victor Macedo Paes Universidade Estadual do Ceará, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza-CE, Brazil
  • Francisco Leo Agiar Universidade Estadual do Ceará, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza-CE, Brazil
  • Juliana Jales de Hollanda Celestino Universidade da Integração Internacional da Lusofonia Afro-Brasileira, Institute of Health Sciences. Acarape-CE, Brazil
  • Benner Geraldo Alves Universidade Estadual do Ceará, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza-CE, Brazil
  • Otilia Deusdenia Loiola Pessoa Universidade Federal do Ceará, Department of Organic and Inorganic Chemistry., Centro de Ciências. Fortaleza-CE, Brazil
  • Ricardo Toniolli Universidade Estadual do Ceará, Laboratory of Swine Reproduction and Semen Technology. Fortaleza-CE, Brazil
  • Ana Paula Rodrigues Universidade Estadual do Ceará, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza-CE, Brazil
  • José Ricardo de Figueiredo Universidade Estadual do Ceará, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA). Fortaleza-CE, Brazi

DOI:

https://doi.org/10.32480/rscp.2019-24-2.274-292

Keywords:

Auxemma oncocalyx, Oncocalyxone A, Doxorubicin, in vitro maturation, in vitro fertilization, COCs, embryo development

Abstract

Most anticancer drugs like doxorubicin (DXR) have low specificity that results in undesirable effects especially when it comes to collateral effects on reproduction. Plants are excellent sources when searching for new drugs. Auxemma oncocalyx (A. oncocalyx) and its main component Oncocalyxone A (onco A) have anti-tumoral activity and are less toxic than DXR in reproductive parameters. However, there are no studies on the action of these drugs regarding the porcine in vitro oocyte competence and embryo development. The aim of this study was to evaluate the effect of A. oncocalyx and onco A exposure during in vitro maturation (IVM) of oocytes (Experiment 1) or in vitro embryo culture (IVC) (Experiment 2) on the oocyte developmental competence. For experiment 1, COCs were distributed in IVM medium alone (control) or supplemented with DXR (0.3 ?g/mL), A. oncocalyx (1.2 ?g/mL) and onco A (1 ?g/mL). Then, oocytes were submitted to in vitro fertilization (IVF) and in vitro embryo culture. For experiment 2, zygotes were cultured with DXR, A. oncocalyx and onco A for 7 days. Viability, maturation, fertilization and embryo developmental parameters were evaluated in both experiments. In experiment 1; DXR, A. oncocalyx and onco A reduced (P<0.05) oocyte viability  and  IVM  efficiency.  Onco A increased (P<0.05) the meiotic resumption. After IVF, all drugs reduced (P<0.05) viability, IVF efficiency and percentage of cleaved embryos, nevertheless, only DXR decreased the percentage of blastocyst. In experiment 2; all drugs reduced (P<0.05) the percentage of penetration, but only DXR and onco A decreased (P<0.05) IVF efficiency. DXR and A. oncocalyx decreased (P<0.05) the percentage of cleaved embryo, but had no effect on blastocyst formation. In conclusion, the addition of DXR during IVM or IVC negatively affected the IVF efficiency and cleavage rate. In addition, the exposure of COCs to DXR only during IVM was more detrimental to oocyte viability and blastocyst formation than A. oncocalyx and onco A.

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References

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Published

2019-12-30

Issue

Vol. 24 No. 2 (2019): July-December

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Original Article

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How to Cite

1.
Analysis of the activity of oncocalyxone A (Auxemma oncocalyx) and doxorubicin on the in vitro development of porcine oocytes. Rev. Soc. cient. Py. [Internet]. 2019 Dec. 30 [cited 2025 Sep. 24];24(2):274-92. Available from: https://sociedadcientifica.org.py/ojs/index.php/rscpy/article/view/78

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