Inoculation of soybean (Glycine max (L.) Merr.) with plant growth–promoting rhizobacteria and mycorrhizal fungi under controlled conditions in a soil from Alto Paraná
DOI:
https://doi.org/10.32480/rscp.2026.e3128Keywords:
inoculation, plant growth–promoting rhizobacteria, arbuscular mycorrhizal fungi, soybeanAbstract
Nitrogen (N) and phosphorus (P) are essential macronutrients for plant growth and productivity; however, they are not always available to plants in the soil. The availability of these nutrients can be enhanced through the use of plant growth-promoting bacteria (PGPB) in combination with mycorrhizal fungi, which contribute to biological nitrogen fixation and phosphorus solubilization. This study aimed to evaluate the influence of plant growth-promoting bacteria (PGPB) and mycorrhizal fungi inoculation on soybean production under protected conditions. The experiment was conducted using a completely randomized design with six treatments: T1, uninoculated control; T2, PGPB 1 (Bradyrhizobium japonicum, Azospirillum brasilense, and Pseudomonas fluorescens); T3, PGPB 2, differing from PGPB 1 in the concentration of colony-forming units; T4, arbuscular mycorrhizal fungi (AMF); T5, PGPB 1 (Bradyrhizobium japonicum, Azospirillum brasilense, and Pseudomonas fluorescens) + arbuscular mycorrhizal fungi (AMF); and T6, PGPB 2 + arbuscular mycorrhizal fungi (AMF). Each treatment had four replicates, resulting in a total of 24 experimental units. Soybean seeds (Glycine max (L.) Merr.), RR1 variety, were inoculated and sown in pots containing soil collected from Yguazú, Alto Paraná, Paraguay. The experiment was conducted under controlled conditions in San Lorenzo, Central Department, Paraguay. The variables evaluated were plant height, number of pods, shoot dry mass, root length, root dry mass, number of nodules per plant, and nodule dry mass per plant. The results were statistically analyzed using analysis of variance. No statistically significant differences among treatments were observed for any of the evaluated variables. The mean values were 46 cm for plant height, 11 pods per plant, 47 cm for root length, 14 g and 3 g for shoot and root dry mass, respectively, and 17 nodules per plant, with a nodule dry mass of 1 g per plant. Under the experimental conditions, the use of plant growth-promoting bacteria and mycorrhizal fungi resulted in a limited response that was not sufficiently pronounced to be statistically significant.
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