Air quality assessment using the Pollen Abortion assay in Tradescantia pallida in a Mid-sized City in Southern Brazil
DOI:
https://doi.org/10.32480/rscp.2021.26.1.6Keywords:
air pollution, biomonitoring, pollen, genotoxicityAbstract
Urbanization is an important source of air pollutants that can compromise human health. In developing countries, such as Brazil, most cities do not have air quality monitoring stations. Assessing air quality through plant species has gained recognized prominence, as they are sessile organisms and sensitive to environmental changes. Pollen abortion assay in Tradescantia pallida is a fast and low-cost bioassay that can be implemented in passive biomonitoring scenarios. The present study aimed to use the pollen abortion assay in T. pallida to assess air quality in the municipality of Rio Grande, RS, Brazil and the possible relationship with vehicular flow. A relation was found between the highest rate of pollen abortion and the sites where there was greater vehicular flow and ozone levels, while at the control point, the lowest rate of pollen abortion among the others was found, corroborating the hypothesis that air pollution together with high levels of ozone from vehicles, impair plant pollination.
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References
1. World Health Organization. Ambient air pollution: A global assessment of exposure and burden of disease. 2016.
2. Akimoto H. Global Air Quality and Pollution. Science. 2003;302(5651):1716-1719.
3. Brasil. Resolução CONAMA Nº 491/2018 - Dispõe sobre padrões de qualidade do ar. [Internet]. 2018 [cited 30 September 2020]. Available from: http://www2.mma.gov.br/port/conama/legiabre.cfm?codlegi=740
4. World Health Organization. WHO air quality guidelines for particulate matter, ozone, nitrogen dioxide and sulfur dioxide. 2005 [cited 30 September 2020]. Available from: http://whqlibdoc. who.int/hq/2006/WHO_SDE_PHE_OEH_06.02_eng.pdf.
5. Lindén J, Boman J, Holmer B, Thorsson S, Eliasson I. Intra-urban air pollution in a rapidly growing Sahelian city. Environment International. 2012;40:51-62.
6. Réquia W, Koutrakis P, Roig H. Spatial distribution of vehicle emission inventories in the Federal District, Brazil. Atmospheric Environment. 2015;112:32-39.
7. Greguskova E, Mi?ieta K. Phytoindication of the ecogenotoxic effects of vehicle emissions using pollen abortion test with native flora. Pol Journal Environmental Study. 2013;22:1069-1076.
8. Mišík M, Mi?ieta K, Solenská M, Mišíková K, Pisar?íková H, Knasmüller S. In situ biomonitoring of the genotoxic effects of mixed industrial emissions using the Tradescantia micronucleus and pollen abortion tests with wild life plants: Demonstration of the efficacy of emission controls in an eastern European city. Environmental Pollution. 2007;145(2):459-466.
9. Carneiro M, Ribeiro F, Fernandes-Filho F, Lobo D, Barbosa F, Rhoden C et al. Pollen abortion rates, nitrogen dioxide by passive diffusive tubes and bioaccumulation in tree barks are effective in the characterization of air pollution. Environmental and Experimental Botany. 2011;72(2):272-277.
10. Carvalho-Oliveira R, Amato-Lourenço L, Moreira T, Silva D, Vieira B, Mauad T et al. Effectiveness of traffic-related elements in tree bark and pollen abortion rates for assessing air pollution exposure on respiratory mortality rates. Environment International. 2017;99:161-169.
11. de Oliveira Alves N, de Souza Hacon S, de Oliveira Galvão M, Simões Peixotoc M, Artaxo P, de Castro Vasconcellos P et al. Genetic damage of organic matter in the Brazilian Amazon: A comparative study between intense and moderate biomass burning. Environmental Research. 2014;130:51-58.
12. Santos A, Segura-Muñoz S, Nadal M, Schuhmacher M, Domingo J, Martinez C et al. Traffic-related air pollution biomonitoring with Tradescantia pallida (Rose) Hunt. cv. purpurea Boom in Brazil. Environmental Monitoring and Assessment. 2015;187(2).
13. Krupa S, Manning W. Atmospheric ozone: Formation and effects on vegetation. Environmental Pollution. 1988;50(1-2):101-137.
14. Wilkinson S, Mills G, Illidge R, Davies W. How is ozone pollution reducing our food supply?. Journal of Experimental Botany. 2011;63(2):527-536.
15. Fleck A, Vieira M, Amantéa S, Rhoden C. A Comparison of the Human Buccal Cell Assay and the Pollen Abortion Assay in Assessing Genotoxicity in an Urban-Rural Gradient. International Journal of Environmental Research and Public Health. 2014;11(9):8825-8838.
16. Brasil. Instituto Brasileiro de Geografia e Estatística. 2020 [cited 30 September 2020]. Available from: https://www.ibge.gov.br/cidades-e-estados/rs/rio-grande.html
17. Garcia F, Mirlean N, Baisch P. Marcadores metálicos como avaliação do impacto crônico de emissões petroquímicas em zona urbana. Química Nova. 2010;33(3):716-720.
18. da Silva Júnior F, Feijo Fernandes C, Tavella R, Hoscha L, Martins Baisch P. Genotoxic damage in coelomocytes of Eisenia andrei exposed to urban soils. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2019;842:111-116.
19. Honscha L, de Moura R, Baisch P, Da Silva Júnior F. Increasingly Distant from Eden—a Look at the Soils of Protected Areas Using Ecotoxicological Tests and Chemical Analysis. Water, Air, & Soil Pollution. 2019;230(7)..
20. Gutierrez F, Eslava Martins S, Honscha L, de Lima Brum R, Vargas V, Mirlean N et al. Is There Something in the Air? Sources, Concentrations and Ionic Composition of Particulate Matter (PM2.5) in an Industrial Coastal City in Southern Brazil. Water, Air, & Soil Pollution. 2020;231(5).
21. da Silva Júnior F, Honscha L, Brum R, Ramires P, Tavella R, Fernandes C et al. Air quality in cities of the extreme south of Brazil. Ecotoxicology And Environmental Contamination. 2020;15:61-67.
22. IBGE. Instituto Brasileiro de Geografia e Estatística . Censo brasileiro de 2010. Rio de Janeiro: IBGE, 2012.
23. Brasil. Denatran. - Frota de veículos 2019. 2019 [cited 30 September 2020]. Available from: https://infraestrutura.gov.br/component/content/article/115-portal-denatran/8559-frota-de-veiculos-2019.html.
24. Mi?ieta K, Murín G. Microspore analysis for genotoxicity of a polluted environment. Environmental and Experimental Botany. 1996;36(1):21-27.
25. Mišík M, Solenská M, Mi?ieta K, Mišíková K, Knasmüller S. In situ monitoring of clastogenicity of ambient air in Bratislava, Slovakia using the Tradescantia micronucleus assay and pollen abortion assays. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2006;605(1-2):1-6.
26. Fleck A, Moresco M, Rhoden C. Assessing the genotoxicity of traffic-related air pollutants by means of plant biomonitoring in cities of a Brazilian metropolitan area crossed by a major highway. Atmospheric Pollution Research. 2016;7(3):488-493.
27. Solenská M, Mi?ieta K, Mišík M. Plant Bioassays for an in Situ Monitoring of Air Near an Industrial Area and a Municipal Solid Waste – ŽILINA (SLOVAKIA). Environmental Monitoring and Assessment. 2006;115(1-3):499-508.
2. Akimoto H. Global Air Quality and Pollution. Science. 2003;302(5651):1716-1719.
3. Brasil. Resolução CONAMA Nº 491/2018 - Dispõe sobre padrões de qualidade do ar. [Internet]. 2018 [cited 30 September 2020]. Available from: http://www2.mma.gov.br/port/conama/legiabre.cfm?codlegi=740
4. World Health Organization. WHO air quality guidelines for particulate matter, ozone, nitrogen dioxide and sulfur dioxide. 2005 [cited 30 September 2020]. Available from: http://whqlibdoc. who.int/hq/2006/WHO_SDE_PHE_OEH_06.02_eng.pdf.
5. Lindén J, Boman J, Holmer B, Thorsson S, Eliasson I. Intra-urban air pollution in a rapidly growing Sahelian city. Environment International. 2012;40:51-62.
6. Réquia W, Koutrakis P, Roig H. Spatial distribution of vehicle emission inventories in the Federal District, Brazil. Atmospheric Environment. 2015;112:32-39.
7. Greguskova E, Mi?ieta K. Phytoindication of the ecogenotoxic effects of vehicle emissions using pollen abortion test with native flora. Pol Journal Environmental Study. 2013;22:1069-1076.
8. Mišík M, Mi?ieta K, Solenská M, Mišíková K, Pisar?íková H, Knasmüller S. In situ biomonitoring of the genotoxic effects of mixed industrial emissions using the Tradescantia micronucleus and pollen abortion tests with wild life plants: Demonstration of the efficacy of emission controls in an eastern European city. Environmental Pollution. 2007;145(2):459-466.
9. Carneiro M, Ribeiro F, Fernandes-Filho F, Lobo D, Barbosa F, Rhoden C et al. Pollen abortion rates, nitrogen dioxide by passive diffusive tubes and bioaccumulation in tree barks are effective in the characterization of air pollution. Environmental and Experimental Botany. 2011;72(2):272-277.
10. Carvalho-Oliveira R, Amato-Lourenço L, Moreira T, Silva D, Vieira B, Mauad T et al. Effectiveness of traffic-related elements in tree bark and pollen abortion rates for assessing air pollution exposure on respiratory mortality rates. Environment International. 2017;99:161-169.
11. de Oliveira Alves N, de Souza Hacon S, de Oliveira Galvão M, Simões Peixotoc M, Artaxo P, de Castro Vasconcellos P et al. Genetic damage of organic matter in the Brazilian Amazon: A comparative study between intense and moderate biomass burning. Environmental Research. 2014;130:51-58.
12. Santos A, Segura-Muñoz S, Nadal M, Schuhmacher M, Domingo J, Martinez C et al. Traffic-related air pollution biomonitoring with Tradescantia pallida (Rose) Hunt. cv. purpurea Boom in Brazil. Environmental Monitoring and Assessment. 2015;187(2).
13. Krupa S, Manning W. Atmospheric ozone: Formation and effects on vegetation. Environmental Pollution. 1988;50(1-2):101-137.
14. Wilkinson S, Mills G, Illidge R, Davies W. How is ozone pollution reducing our food supply?. Journal of Experimental Botany. 2011;63(2):527-536.
15. Fleck A, Vieira M, Amantéa S, Rhoden C. A Comparison of the Human Buccal Cell Assay and the Pollen Abortion Assay in Assessing Genotoxicity in an Urban-Rural Gradient. International Journal of Environmental Research and Public Health. 2014;11(9):8825-8838.
16. Brasil. Instituto Brasileiro de Geografia e Estatística. 2020 [cited 30 September 2020]. Available from: https://www.ibge.gov.br/cidades-e-estados/rs/rio-grande.html
17. Garcia F, Mirlean N, Baisch P. Marcadores metálicos como avaliação do impacto crônico de emissões petroquímicas em zona urbana. Química Nova. 2010;33(3):716-720.
18. da Silva Júnior F, Feijo Fernandes C, Tavella R, Hoscha L, Martins Baisch P. Genotoxic damage in coelomocytes of Eisenia andrei exposed to urban soils. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2019;842:111-116.
19. Honscha L, de Moura R, Baisch P, Da Silva Júnior F. Increasingly Distant from Eden—a Look at the Soils of Protected Areas Using Ecotoxicological Tests and Chemical Analysis. Water, Air, & Soil Pollution. 2019;230(7)..
20. Gutierrez F, Eslava Martins S, Honscha L, de Lima Brum R, Vargas V, Mirlean N et al. Is There Something in the Air? Sources, Concentrations and Ionic Composition of Particulate Matter (PM2.5) in an Industrial Coastal City in Southern Brazil. Water, Air, & Soil Pollution. 2020;231(5).
21. da Silva Júnior F, Honscha L, Brum R, Ramires P, Tavella R, Fernandes C et al. Air quality in cities of the extreme south of Brazil. Ecotoxicology And Environmental Contamination. 2020;15:61-67.
22. IBGE. Instituto Brasileiro de Geografia e Estatística . Censo brasileiro de 2010. Rio de Janeiro: IBGE, 2012.
23. Brasil. Denatran. - Frota de veículos 2019. 2019 [cited 30 September 2020]. Available from: https://infraestrutura.gov.br/component/content/article/115-portal-denatran/8559-frota-de-veiculos-2019.html.
24. Mi?ieta K, Murín G. Microspore analysis for genotoxicity of a polluted environment. Environmental and Experimental Botany. 1996;36(1):21-27.
25. Mišík M, Solenská M, Mi?ieta K, Mišíková K, Knasmüller S. In situ monitoring of clastogenicity of ambient air in Bratislava, Slovakia using the Tradescantia micronucleus assay and pollen abortion assays. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2006;605(1-2):1-6.
26. Fleck A, Moresco M, Rhoden C. Assessing the genotoxicity of traffic-related air pollutants by means of plant biomonitoring in cities of a Brazilian metropolitan area crossed by a major highway. Atmospheric Pollution Research. 2016;7(3):488-493.
27. Solenská M, Mi?ieta K, Mišík M. Plant Bioassays for an in Situ Monitoring of Air Near an Industrial Area and a Municipal Solid Waste – ŽILINA (SLOVAKIA). Environmental Monitoring and Assessment. 2006;115(1-3):499-508.
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2021-06-10
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1.
Air quality assessment using the Pollen Abortion assay in Tradescantia pallida in a Mid-sized City in Southern Brazil. Rev. Soc. cient. Py. [Internet]. 2021 Jun. 10 [cited 2026 May 2];26(1):6-16. Available from: https://sociedadcientifica.org.py/ojs/index.php/rscpy/article/view/176
